الفهرس 
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Abstract
This study aimed to isolate, characterize, and identify some indigenous bacteria from different sources known to be contaminated with pesticides and evaluate their ability to degrade two important pesticides, chlorantraniliprole (CAP), and flubendiamide (FBD). Six isolates showed maximum growth in the presence of CAP and FBD in the growth medium as a sole carbon source. The isolates were identified by biochemical and morphological tests, as well as MALD- TOF-MS, and 16S rRNA techniques. The study have shown that the isolated microbes were identified as Bacillus subtilis subsp. subtilis AZFS3, Bacillus pumilus AZFS5, Bacillus mojavensis AZFS15, Bacillus paramycoides AZFS18, Pseudomonas aeruginosa KZFS4, and Alcaligenes aquatilis KZFS11. The degradation ability of the studied bacterial strains against diamide pesticides was estimated under different conditions (temperature degree, pH, salinity, and incubation period) for optimization of biodegradation process. The results revealed that the optimal conditions of growth all bacterial strains’ were 30–35 °C, pH 7.0, 0.0–0.5% NaCl, and an incubation period of 11 days and shaking at 150 rpm in the presence of diamide insecticides at 50 mg/L. The capacity of six bacterial strains to CO2 production and degradation ability against various diamide pesticides and other pesticide groups (Profenofos, Cypermethrin,
Carbofuran, and Malathion) were evaluated. The results showed that the Pseudomonas aeruginosa KZFS4 (LC599404.1) strain produced the highest CO2 evolution, about 1.226 mg CO2/16 days, with efficacy in the biodegradation of FBD-CAP 78.6 %while the absorbance of bacterial growth (OD 600) on various pesticides ranged from
1.542 to 1.701. Additionally, Consortium-(No. 3)-mix-6- strains gave 1.553 mg CO2/16 days with efficacy 99.6 % and turbidity of 2.122 to 2.365 (OD 600) on various pesticides. In conclusion, the six bacterial strains could play an important role in the biodegradation process of pollutants, especially pesticides in soils.