الفهرس 
Chapter (1) : Anatomy and Physiology of the SkinOnly 14 pages are availabe for public view
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Abstract
Surgical methods in the management of leucoderma (both vitiliginous and nonvitiliginous) have a well-documented and established role in the management of resistant cases. The basic principle of all surgical methods is transfer of melanocytes from uninvolved skin into a stable leucoderma lesion, where they grow into, and function as, effective epidermal-melanin units. Although noncellular grafts such as, split thickness, suction blister, and punch grafts are the mainstay of surgical management, a number of cellular methods have become increasingly popular in recent times.
Surgical techniques have proven to be effective in stable cases, and melanocyte transfer may be performed by punch minigrafting, split-thickness skin grafting, hair follicle transplantation, suction blisters, epidermal curettage techniques and cultured and non-cultured autologous melanocytes. The previous surgical and cultured techniques can be time-consuming and, in some cases, aesthetically unsatisfying or painful for the patients.
In recent years, new non-cultured autologous epidermal techniques have been developed with similar results to the cultured melanocytes techniques, but are simpler, less expensive and less timeconsuming. These techniques do not require the use of a laboratory but only a split-thickness thin biopsy that is pre-treated with trypsin solution.
Cellular grafts include different methods such as, noncultured basal cell enriched melanocyte suspension, cultured mixed melanocyte-keratinocyte suspension (with or without carrier) , and cultured pure melanocyte suspension .
Indications for cellular grafts are similar to those used for tissue methods. The commonest indication is stable vitiligo, refractory to standard medical and physical modalities of treatment. It is particularly useful as a primary treatment for segmental vitiligo and focal vitiligo.
This study aimed to evaluate the applicability of cell therapy using non cultured epidermal transplantation regarding the outcome, obstacles and possible complications in treatment of post burn hypopigmentation.
This was a study which included 35 cases with post burn hypopigmentation who need managment of this hypopigmentation admitted to Plastic & Reconstructive Surgery Department in Menoufia University Hospitals. from april 2020 to the end of study and follow up of patients.
The main results of the study revealed that:
The current study included 35 patients who had burn, their age ranged between 19 – 49 years with mean value of 32.40 ± 7.46. 40% were females and 60% were males. the preoperative dyspigmentation ranged between 6.2 – 24.5 cm3 with mean value of 14.2 ± 4.65 and the postoperative dyspigmentation ranged between 2.36 – 16.17 cm3. The Repigmentation% ranged between 20-95% and the color matching% ranged between 30-80%. Most of cases had good re-pigmentation 60% while 22.9% had fair re-pigmentation. Excellent re-pigmentation was detected in 14.3% while 2.9% had poor re-pigmentation. According to Kahn grading system, 40% were poor, 28.6% were fine, 25.7% were good and 5.7% were excellent.
Complications occurred in 28.6% of cases that was in the form of atrophy in 14.3%, scare in 11.4% and infection in 2.9%. There is significant association between the development of complications and the site of burn (more in face & neck) , presence of obstacles and the severity of burn (more in full thickness than partial thickness burn) . There is statistically significant association between the development of complications and poor outcome in the studied cases. There is no statistically significant association between the presence of obstacles and poor outcome in the studied cases. There is no significant correlation between the repigmentation% and the clinical data of the studied population