الفهرس 
Aim of The WorkOnly 14 pages are availabe for public view
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Abstract
Colorectal carcinoma (CRC) is the third most common cancer in the world, constituting about 10% of all cancers.
In Egypt, according to Pathology-Based Cancer Registry, Ain-Shams University, CRC constituted 8.6% of all malignancies and 41.5% of malignant digestive system tumors.
The aim of this work is to evaluate Aldehyde dehydrogenase 1A1 (ALDH1A1) expression in colorectal adenocarcinoma and determine the relation between ALDH1A1 expression and different clinico-pathological variables.
This is a selected retrospective study included 50 different cases of colorectal adenocarcinoma. The material included archival formalin fixed paraffin embedded blocks, processed during the years 2015-2020 and collected from Pathology Department and Early Cancer Detection Unit; Faculty of Medicine, Benha University, Egypt.
The clinico-pathological data were tabulated and analyzed using the computer program SPSS (Statistical package for social science).
Comparison between histopathological subtypes according to the grade and stage of studied cases was performed. It revealed a highly significant statistical difference (P value < 0.001 and = 0.005 respectively). Comparison between the grades regarding the stage of studied cases was also performed and revealed no significant statistical difference (P value
= 0.059).
ALDH1A1 has been used as a stem cell marker, and plays important functional roles in stem cells. Expression of ALDH1A1 by cancer stem cells has been demonstrated in multiple types of cancers.
ALDH1A1 immunostaining was performed for each case. Positivity was considered as brownish homogenous cytoplasmic staining of tumor cells. The immunohistochemical scores were obtained by light microscopy as the staining intensity (scored from 0–3) multiplied by the percentage area of positive immunostaining within the visual field (scored from 0–4). The cut-off value for high versus low expression of the ALDH1A1 protein was determined using receiver-operating characteristic (ROC) curve analysis and SPSS statistical software, defining a final immunostaining score of >3.5 as high ALDH1A1 protein expression.
Out of 50 cases of colorectal adenocarcinoma, 24 cases (48%) showed low ALDH1A1 expression and 26 cases (52%) showed high expression.
Statistical analysis was performed on the relation between ALDH1A1 expression in studied CRC cases and clinico-pathological variables. It revealed a highly significant statistical difference between ALDH1A1 expression in studied cases according to tumor site, lymph node metastasis and TNM stage (P value = 0.007, 0.001 and <0.001 respectively). It also revealed a significant statistical difference according to lymphovascular and perineural invasion (P value = 0.011 and 0.013 respectively). Other clinico-pathological variables showed no significant statistical difference (P value >0.05).