الفهرس 
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Abstract
Since the 100s of last century, many benzodiazepine derivatives have provoked wide interest for their significant biological and pharmaceutical activities, such as antiepileptic, and tranquilizer. The current interest in benzodiazepine derivatives is due to the treatment of anxiety, insomnia, and sleep disorders as well as sedative, hypnotic, muscle relaxant, and anticonvulsant. The main aim of the present thesis is focused on the preparation of new benzodiazepine derivatives. Their structures are elucidated by means of elemental analysis, spectroscopic (IR, UV-visible, EI-mass and 1H NMR). Also, apply these reactions in mapping different benzodiazepine in plasma, pharmaceuticals and in biotic fluids as well as study new method for extraction of benzodiazepine derivatives. Furthermore, the study is extended to include the fluorescence sensing of benzodiazepine derivatives. Finally, the effect on the anticonvulsant activity of benzodiazepine derivatives were carried out on male Swiss albino mice. A brief description of the overall work in this thesis can be summarized as follows: Chapter one displays different publications concerning the benzodiazepine derivatives with great attention to the compounds like that investigated and their biological activities. The second chapter covers the experimental part and illustrates the experiments used in this work. It includes a list of reagents along with the physical measurements and instrumentation, as well as the working procedures and a description for the preparation of the benzodiazepine derivatives. In addition, this chapter includes the recommended procedures for the anticonvulsant activity. three deals with the results and discussion which are summarized to give the following information: 1- The proposed chemical formulae of the benzodiazepine derivatives are in good agreement with the stoichiometries concluded from their analytical data of carbon, hydrogen, and nitrogen contents. 2- Most benzodiazepine derivatives have melting points (90-290˚C). 3- IR, UV-visible, EI-mass and 1H NMR were used to confirm the proposed structures of benzodiazepine derivatives. 4- The detection limit and limit of quantification of bromazepam using 2,4,6-trichloro-1,3,5-triazine are evaluated by fluorescence. The values of detection limit and limit of quantification are 0.135 and 0.409 μg/ml, respectively, for bromazepam as well as 0.263 ,0.799 μg/ml, respectively for clonazepam, suggesting that the 2,4,6-trichloro-1,3,5-triazine is suitable as sensitive detection probe for bromazepam and clonazepam. 5- The final part in this chapter deals with the anticonvulsant activity for derivatives of bromazepam and clonazepam.