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Abstract Cellulose is a sustainable resource on earth. With the development of nanotechnology, the interest of cellulose scientists has moved to the separation of nanocellulose their importance in technical applications and its usage in detection of pollutants. For the analytical determination of Fe (II) and Pd(II) concentrations, a novel optical sensor based on spectro-photometric technique was used. It was prepared by direct immobilization of 5-amino-phenanthrolin 3formylsalicylicacid,onto nanocellulose. Fe(II) detection and quantification limits of 0.239 and 0.796 ppb and for Pd(II) were 0.318 ppb and 1.06 ppb, respectively. The results demonstrated that the 5-Phen sensor could measure Fe(II) in human blood serum accurately. |