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Abstract It’s estimated that there are between 350,000 and almost 500,000 vascular plants species, the majority of which are employed as medicinal plants. Traditional medicine draws on a long history of using plants as sources of health benefits. In the past, people would treat their illnesses or even merely try several remedies until they found one that worked. Often referred to as ”traditional medicine,” the use of these herbs has been refined over many centuries. Officially, traditional medicine is ” of different cultures’ knowledge, practices and skills based on their beliefs, theories and local experience that are employed in maintaining health and avoiding, identifying, improving or curing both mental and physical illness” [102]. Biologically active chemicals, such as secondary metabolites, extracted from plants are of tremendous utility in treatment of numerous human diseases [128]. The pharmaceutical and medicinal industries gain significant benefits from the secondary metabolite, and plant and cell cultures that provide an efficient and cost-effective method of triggering the bulk production of these compounds. [11]. As compared to traditional methods, plant multiplication can be considerably sped up by the use of in vitro propagation that operating underneath a bioreactor micropropagation system in order to increase the reproduction rates of in vitro-grown shoots [26]. So The use of in vitro culture methods offers a way out of the difficulties of field production, which in turn helps to preserve the plants [79]. |