الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
The current study focuses on the identification of the diagnostic together with significance for prognosis caused by Notch-2 gene mutations in DLBCL cases. Furthermore, the current work evaluates molecular expression of Notch-2 gene mutations in ABC as well as GCB subtypes of DLBCL.
20 individuals with newly diagnosed DLBCL who were hospitalised at hematology department participated in the current study together with those who attended hemato-oncology OPD) Alexandria Hospital (MUH), beside 10 healthy individuals as a control.
Lymph node biopsies were conducted on each subject. For staging reasons, a radiological assessment was performed initially. Four rounds of chemotherapy were then followed by another evaluation to determine the medication’s efficacy. A complete clinical examination and history interview were conducted for each candidate. Prior to starting therapy, the ECOG scores of each patient were obtained.
Applying new classification from WHO, DLBCL was identified with respect to their histology as well as IHC followed by classification into those 2 molecular subtypes as GCB/ABC. Using antibodies for CD-10 and MUM-1 as well as BCL6, IHC was performed, and Han’s Algorithm was used to classify patients into appropriate molecular groupings. 40 percent of cases (8 patients) identified as Germinal Centre subtype while 60 percent equal to 12 cases were subtype Activated B cell.
All patients received RCHOP therapy, which was repeated every 21 days. Subsequent four RCHOP rounds, subjects had a 2nd assessment for verification of response.
In the present investigation, 20 patients with newly diagnosed DLBCL and 10 healthy participants’ clinical and nucleotide sequencing data for Notch-2 mutation were analysed. The study included the medical history, clinical information, laboratory results, IHC, and sequencing analysis of Notch-2 gene mutations for DLBCL patients.
The current study found that DLBCL patients had a mean diagnostic age of 47 years at diagnosis, with 65 percent of men and 35 percent of women. Fever (65 percent) and night sweats (35 percent), weight loss (25 percent), and weariness (20 percent) were the most common clinical manifestations. Additionally, patients showed hepatomegaly of 5% and splenomegaly of 20%.
Commonly nodal involvement locations observed were cervical by 65 percent then Axillary in 35 percent as well as inguinal by 25 percent. An average Hb of 11.82 gram/dl reported within the study group of DLBCL cases. On the other hand, 7.46 x10³ per microliter of WBC as well as 270 x103 per microliter of Mean Platelet counts observed.