الفهرس 
Microbial pigmentsOnly 14 pages are availabe for public view
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Abstract
MRSA, antibacterial, pyocyanin, antibiofilm, anti-quorum sensing, antivirulence agent, anticancer, human breast adenocarcinoma (MCF-7)Pyocyanin, a specific extracellular secondary metabolite pigment produced by Pseudomonas aeruginosa, exhibits redox activity and has toxic effects on bacterial and mammalian cells, making it a new antibacterial agent and a potent alternative for treating cancer. Hence, the present study was performed to explore the antibacterial effect of purified and characterized pyocyanin against methicillin-resistant Staphylococcus aureus (MRSA) as well as its effect on pre-established MRSA biofilm and quorum sensing (QS). Additionally, the anticancer activity of pyocyanin was examined against the human breast adenocarcinoma MCF-7. The maximum production of pyocyanin (53 μg/ml) was achieved by incubating the highest pyocyanin-producing P. aeruginosa isolate (P 32) in pH-adjusted peptone water supplemented with 0.3% (w/v) cetrimide under shaking conditions at 37°C for 3 days. The high purity of the extracted pyocyanin was confirmed by HPLC against standard pyocyanin. The stability of pyocyanin was affected by the solvent in which it was stored. Therefore, the purified pyocyanin extract was lyophilized to increase its shelf life by up to one year.