الفهرس 
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Abstract
This work aimed to investigate the effect of Nano-Se supplementation and in-ovo injection on physiological responses and productive performance of Dokki- 4 laying breeder hens and post-hatch chicks reared under hot conditions.
A total number of 60 laying hens and 8 cocks Doki-4 strain (74 weeks old and average body weight of female and male 1663.75±34.9 g and 2383.33±147.0, respectively) were randomly divide into two equal groups (30 hens and 4 cocks of each). The first group considered as parents of treatment 1 (Tr1) and treatment 2 (Tr2). The second group considered as parents of treatment 3 (Tr3) and treatment 4 (Tr4). After four weeks of start experiment, 300 fertile eggs collected from two groups (150 eggs / group), the fertile eggs and post-hatch chicks treated as following;
Tr1, parents and post-hatch chicks fed on basal diet plus in- ovo injection with 0.5 ml saline solution /egg (control treatment).Tr2, parents and post-hatch chicks fed on basal diet plus in- ovo injection with 0.5 ml saline solution (containing 0.2 µg Nano –Se) /egg. Tr3, parents fed with basal diet supplemented with 0.3 mg Nano –Se /kg feed and post-hatch chicks fed on basal diet plus in- ovo injection with 0.5 ml saline solution (containing 0.2 µg Nano –Se)/ egg. Tr4, parents and post-hatch chicks fed on basal diet supplemented with 0.3 mg Nano –Se /kg feed plus in- ovo injection with 0.5 ml saline solution (containing 0.2 µg Nano –Se) / egg.
The results showed that, for Dokki- 4 laying breeder hens, There were insignificant differences in red blood cells counts (RBC’s), white blood cells counts (WBC’s) and mean corpuscular hemoglobin concentration (MCHC), hemoglobin (Hb), mean corpuscular hemoglobin (MCH), mean corpuscular volume (MCV) and Heterophils / lymphocyte ratio (H/L ratio) as affected by Nano-se supplementation. On the other hand, hematocrit (Ht, %) significantly (P<0.05) increased in-group 2 (G2) compared with group 1 (G1).
There were insignificant differences in Total protein (TP), Albumin (ALB), Globulin (GLO) and Creatinine (Cr), Aspartate transferase (AST) and Triglycerides (Tri) between G1 and G2 (Nano-selenium supplementation), however, Alanine aminotransferase (ALT) concentration significantly (P<0.05) increased in G1 in when compared to G2.
There were insignificant differences in immunological parameters (IgM and IgY) between G1 and G2 (Nano-selenium supplementation). there were insignificant differences in rectal temperature (RT) between G1 and G2 (Nano-selenium supplementation).
Egg production was significantly increased in G2 when compared with G1 (control).
There were insignificant differences in all egg quality traits between G1 and G2 (Nano-selenium supplementation) except shell thickness (ShTh and Haugh unit (HU) which significantly increased in G2 when compared with G1 (Control).
For post-hatch chicks, hemoglobin and mean corpuscular volume significantly (P<0.05) increased in Tr2 and Tr3 in compared to Tr1 (control). Heterophils / lymphocyte ratio and hatchability % significantly (P<0.05) improved in Tr3 and Tr4 in compared to Tr1. Immunoglobulin M significantly (P<0.05) increased in Tr2 and Tr3 in compared to Tr1 and Tr4, rectal temperature significantly (P<0.05) decreased in Tr4 in compared with Tr1, from one day to 6 weeks of age from hatch, Tr3 and Tr4 show better performance in body weight gain, feed intake and feed conversion when compared with Tr1 and Tr2.
We can concluded that, supplementation the diets of parents of Dokki- 4 strain and post-hatch chicks with 0.3 mg Nano Se / kg feed plus in-ovo injection with 0.2 µg Nano Se /egg enhance physiological responses and productive performance laying breeder hens and post-hatch chicks reared under hot conditions.