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Abstract
Microbial contamination of the freshly slaughtered meat contributes significantly to meat deterioration or spoilage. In the present work, the prevalence of Pseudomonas spp in raw beef meat samples was investigated. A total of 320 raw beef meat samples were randomly collected from retail outlets and abattoir in Cairo - Egypt during the periods from June 2013 to January 2014. The samples were collected, labeled and transported with minimum of delay in an ice box (4-5{u00BA}C) to the laboratory. Bacteriological and molecular examination of the collected samples revealed the recovery of 46 isolates of Pseudomonas with a rate of 14.37%. Two species belonging to genus Pseudomonas were recovered and identified,P. fluorescens and P. aeruginosa, which were isolated at a rate of 8.75 and 5.6%, respectively. Studying the effect of organic acids treatment of beef meat samples on removal of Pseudomonas contaminants revealed that using acetic acid or citric acid at concentration of 2% for 2 min induced significant reduction of P. aeruginosa and P. fluorescens to uncountable levels (less than 30 CFU/cm²).