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Abstract
This study carried out at the experimental nursery of the Ornamental Horticulture Department, Faculty of Agriculture, Cairo University, during the successive seasons of 2012 and 2013. The aim of this study was to investigate the effect of chicken manure at rates of 10 and 20 m3/feddan + humic acid at rates of 125 and 250 ppm and their interactions on growth, herb yield, essential oil yield, essential oil constituents, number of glandular hairs and chemical constituents of Ocimum spp. (O. basilicum, O.sanctum and O. citriodorum) plants. The results in both seasons pointed out that the treated plants with chicken manure at 10 m3 (100 g/pot) then sprayed with humic acid at 125 ppm in the second cut of Ocimum species significantly increased plant height, number of branches, leaf area, fresh and dry weights, number of glands and essential oil yield per plant and per feddan compared to control plants (untreated) but O. basilicum gave the highest values.GC.MS analysis of the essential oil of all treatments was performed to study the main constituents of the different Ocimum spp. Twelve components have been identified in the oil. The major components of the essential oil were linalool , estragole and eugenol followed by Ü-citral, Ý-citral , terpinen- 4-ol , trans-Ü-bergamotene ,t-cadinol , trans-caryophyllene , germacrene , methyl eugenol, and nerol