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Abstract
Graft versus host disease (GVHD) is the major complication of allogeneic hematopoietic stem cell transplantation (HSCT) and cytokines are recognized as important mediators of GVHD. Polymorphisms in the regulatory regions of several cytokine genes have been associated with a number of immune diseases as well as post HSCT complications. The aim of this study was the detection of cytokines genes polymorphisms (CGPs) and their relation to post HSCT complications mainly GVHD in Egyptian patients receiving HSCT. The study included 106 patient/donor pairs who received peripheral blood stem cell transplantation (PBSCT) from HLA-matched identical siblings. DNA for cytokine genotyping was available for 105 patients and 98 donors only. Polymerase chain reaction with sequence specific primers (PCR-SSP) technique was used to analyze the following cytokines genotypes: Tumor necrosis factor-alpha (TNF-Ü) ({u2212}308), transforming growth factor beta1 (TGF-Ý1) (codon10 and codon 25), interleukin (IL)-6 ({u2212}174), IL-10 ({u2212}1082, {u2212}819 and {u2212}592) and Interferon {u2013}gamma (IFN-Þ) (+874). from the current study we can conclude that there was no impact of all tested CGPs on development of GVHD or other post HSCT complications.