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Abstract
Extended spectrum Ý-lactamases (ESBLs) and AmpC Ý-lactamase are enzymes produced by a variety of Gram-negative bacteria which confer an increased resistance to commonly used antibiotics and represent a substantial clinical threat. There are currently no standard diagnostic tests available for detection of AmpC. A total of 100 Enterobacteriaceae isolates were collected. ESBL production was screened by using the disc diffusion test and was confirmed by the combination disc diffusion test, screening of AmpC production was done by cefoxitin disc test, disc approximation test and confirmation was done by AmpC disc test. Isolates screened positive for ESBL were investigated for their susceptibility to novel antibiotics (Temocillin, Tigecycline, Colistin and Doripenem) by E-test. Results showed that among 100 Enterobacteriaceae isolates, 45 were screened positive for ESBL-production using the disc diffusion test and 36 were confirmed by the combination disc test. Nine isolates were screened for AmpC-production using the cefoxitin disc test and 5 isolates were confirmed as AmpC producers by AmpC disc test Using MAST D68C set, 35 isolates were ESBL producers, 2 were AmpC producers, one isolate was both ESBL and AmpC producers