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Abstract
Background: The continued production of haematopoietic cells (red blood cells, B lymphocytes, T lymphocytes, natural killer cells, neutrophils, basophils, monocytes, macrophages and platelets) depends directly on the presence of haematopoietic stem cells (HSCs), the ultimate and only source of all these cells. Aim of the work: Generate mature megakaryocytes (MK) and platelets from peripheral blood HSCs in the culture so this study allows autologous transfusion to thrombocytopenic patients with less contamination and complication. Subjects and Methods: This study was conducted on 10 subjects. Patients were selected from those admitted to the Tropical Medicine Department, Theodor Bilharz Research Institute. Subjects under study were classified into 2 groups; 5 patients with chronic liver disease (CLD) and thrombocytopenia (group I). In addition, 5 healthy individuals served as a control group (group II). Results: Evidence of in vitro expansion and differentiation of haematopoietic stem cells into platelets was detected by flow cytometry. Positive expression of platelet markers CD61 in CLD group was 51.60± 15.33%, while CD61 in the control group was 69.40± 11.14%. Positive expression of platelet markers CD42 in CLD group was 70.00± 22.40%, while CD42 in the control group was 91.40± 7.98% after culturing with suitable media for 14 days