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Abstract Background: Fascia is known to be one of the main stabilizers in the body. It plays an important role in musculoskeletal dynamics of various body regions. The role of fascia is not just a passive one, but an active role has been shown. Structural and mechanical changes in fascia may contribute to musculoskeletal dysfunction. However, it is not quite clear how changes in skeletal muscles by strength gain or loss may affect fascial structure. Purpose: To investigate whether fascia structure would change following 5 weeks of strengthening exercise to normal and weakened calf muscles in a rodent animal model. Subjects: Thirty-two adult healthy Wister rats were divided equally into 4 groups, with 8 rats in each group as follows: control, exercise, BTX-A (Botulinum type A toxin induced weakness of calf muscle), and BTX-A (weakened calf) and exercise groups |