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Abstract
The experimental work was performed in Modern Agriculture Co. Pico Tissue Culture lab., during the period of 2012 to 2014. The aim of this work was to present a suitable protocol for propagation of date palm cv. Sewi and selected plant of the Passion fruit (Passiflora edulis) grown at ”Kerdasa” region through tissue culture technique by callus. Date palm shoot tips were used with leaves primordia were sterilized by Clorox at 50% for 20 minutes and Hgcl2 at 0.1% for 10 minutes, and cultured on {u00BE} MS supplemented with 3.0 mg/l isopentyl adenine (2ip) and 2,4-D (2.5, 5.0 or 10.0 mg/l) , 1.5 g/l activated charcoal and 30 g/l sucrose (callus medium). Callus pieces (1mm3) were cultured on medium supplemented with 2,4-D at 2.0 mg/l with kin at 0.5, 1.0 or 2.0 mg/l and medium supplemented with BA at 3.0 + 2ip at 3.0 (mg/l) and 2,4- D at 20.0, 40.0 or 50.0 mg/l (the embryogenic callus medium). The embryogenic callus was cultured on medium supplemented with 0.1 mg/l NAA and 2ip at 0.1, 0.5 or 1.0 mg/l, and medium supplemented with 0.5 mg/l NAA and BA and 2ip at 0.0, 0.5 or 1.0 mg/l (the embryogenesis medium). The initiated embryos were cultured on medium supplemented with IBA at 0.0, 0.1, 0.5 and 1.0 mg/l and 0.5 mg/l BA. High concentrations of 2,4-D increased callus induction