الفهرس 
histological Evaluation of Bone Marrow Derived Stem Cell Therapy on Experimentally Induced Osteoarthritis in Albino Rats’ Knee Joint
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Abstract
This work aims to evaluate the efficacy of intra-articular injection of bone marrow derived - mesenchymal stem cells (MSCs) in treatment of mono - iodoacetate (MIA) induced osteoarthritis in rat knee joint monitored by histological and immunohistochemical methods. This study was carried out on 45 adult male albino rats. They were classified into 4 Groups: group I (control group), group II (osteoarthritic group) in which rats received 1mg of MIA and sacrificed after 2 weeks (in subgroup IIa) and after 4 weeks (in subgroup IIb), group III (stem cell treated group) in which rats received MSCs 2 weeks after MIA injection (in subgroup IIIa) or 4 weeks after MIA (in subgroup IIIb) and sacrificed 2 weeks later and group IV (untreated group) in which rats received PBS 2 weeks after MIA injection (in subgroup IIIa) or 4 weeks after MIA (in subgroup IIIb) and sacrificed 2 weeks later. Sections were taken from rats’ knee joints and stained with Hematoxylin and eosin, toluidine blue, immunohistochemichal stains for collagen type II. Sections were examined by light microscopy and immunofluroscent microscopy for PKH26 - labeled MSCs. The mean articular cartilage (AC) thickness, optical density of cartilage matrix proteoglycan and area percent of collagen type II immunoreactivity were measured using image analyzer and statistically analyzed