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Abstract NFGNB can cause a vast variety of infections. They possesses intrinsic mechanisms of resistance to various groups of drugs, especially to carbapenems, which are widely used in clinical practice in health care settings. The aim of the work is to test carbapenem resistance among non-fermenter gram negative bacilli by using different phenotypic tests and automated methods, and to correlate their results with the susceptibility of isolates to carbapenems. The study was conducted on clinical isolates of NFGNB that were isolated from adult patients different samples sent to microbiology laboratory of NCI, over a period of six months from (October 2012 to March 2013). All the 60 NFGNB were subjected to phenotypic tests to detect carbapenemases and MBL; Modified hodge test, imipinem-EDTA combined disc synergy test. Twenty eight isolates (64.7%) were positive by Modified Hodge test (MHT), out of them, 7 isolates (25%) were pseudomonas aeruginosa, and 21 isolates (75%) were Acinetobacter baumannii |