الفهرس 
Chronic hepatitis COnly 14 pages are availabe for public view
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Abstract
The aim of this work was to to assess the expression of two markers (Arginase-1 and Glutamine Synthetase) in chronic Hepatitis C, liver cirrhosis and, hepatocellular Carcinoma, and correlate their expression with clinico-histopathological data.
Arginase-1, also known as liver type arginase. Arg-1, was found to be expressed in the normal liver with a high degree of specificity. There are many studies investigated its potential utility as an immunohistochemical marker for hepatic differentiation.
Glutamine Synthetase catalyzes the synthesis of glutamine from glutamate and ammonia in the mammalian liver where it has been shown to be restricted to hepatocytes surrounding the terminal hepatic venules in the murine and human liver. It is known that glutamine, the end product of GS activity, is the major energy source of tumor cells. Based on findings on experimental hepatocarcinogenesis.
This retrospective study was carried upon 74 cases liver cases: 17 (23%) cases of chronic hepatitis C, 25 (33.8%) cases of cirrhosis, and 32 (43.2%) cases of conventional Hepatocellular carcinoma (HCC). In addition to 8 cases of apparently normal liver biopsy, as a control group.
All apparently normal liver tissue, showed Arg-1 high expression but showed GS low expression.
In chronic hepatitis c cases, There was no statistically significant relation between scoring of Arg-1 expression and grade of activity (p> 0.05.
In HCC cases, there was positive statistical significant relation between Arg-1 expression and median AFP (p< 0.05), increase score of expression with increase alpha fetoprotein median value.
There was positive statistical significant relation between Arg-1 expression and focal lesions, site and size (p< 0.05).
There was higly positive statistical significant difference between chronic hepatitis C, cirrhosis and HCC group regarding the expression of Arg-1 expression (P<0.001 ), as Arg-1 was highest among chronic hepatitics C group followed by cirrhotic group and the least is for HCC group.
In chronic hepatitis c , there was no statistical significant relation between GS expression and grade of activity (p>0.05).
There was statistically significant positive relation between GS expression and grade of HCC (p = 0.05).
There was highly positive statistical significant difference between chronic hepatitis C, cirrhosis and HCC group regarding the expression of GS (P<0.001 ), as GS expression was zero for most cases with chronic hepatitis C (82.4%) and 17.6% score 1+, for cirrhosis; 92% score 0 and 8% score 1+ and for HCC; 62.5% were score 3+, 34.4% score 2+ and 3.1% score 1+.
There was significant inverse relation between Arg-1 and GS regarding their expression in studied chronic hepatitis C, cirrhosis and HCC group (P<0.001 ).
Using receiver-operating characteristic (ROC), found that GS good marker in differentiating HCC from Cirrhosis and chronic hepatitis C, unlike Arg 1 showed positive expression in HCC, cirrhosis and chronic hepatitis C.