الفهرس | Only 14 pages are availabe for public view |
Abstract Generally, most of the food poisoning outbreaks from seafood appear to be due to shellfish consumption, mainly gandoffli because of its filter feeders pattern. Many microbes used as indicator of hygienic status of several foods; one is Escherichia coli which used to detect fecal pollution in water and shellfish. Therefore, twenty Gandoffli samples randomly collected from local markets at Ismailia city, Egypt, for evaluation of Enterobacteriaceae counts, Identification of E. coli and detection of phoA and Shiga toxin genes. The obtained results revealed that the total enterobacteriaceae count of the gandoffli ranged from 7×102 to 7×105 cfu/g with an average of 5×104 ±3.5×104 cfu/g. The occurrence of enterobacteriacea members in gandoffli were represented by E. coli, Acinetobacter lwoffii, Enterobacter hormaechei, Kelbsiella oxytoca, Stentrophomonas maltophilia, Moraxella lacunata, Achromobacter xylosoxidans, and ESBL E. coli. In addition, the isolated E. coli from Gandoffli were subsequently serologically typed into O103, O55, O 128, O126,O118 and O157 then confirmed using conventional polymerase chain reaction by presence of alkaline phosphatase gene . Upon checking virulence genes in E. coli: stx2 was absent in O157 and O103. Also, stx1 was present in O157 and absent in O103. It could be concluded that Gandoffli samples were exposed to inadequate hygienic measures during harvest, transportation, storage or handling that led to their contamination or bacterial overload from different sources by foodborne pathogens. |