الفهرس 
IL-6 and IL-10 gene polymorphisms and graft versus-host disease in Egyptian children after matched sibling hematopoietic stem cell transplantation
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Abstract
Background/objective: Graft-versus-host disease (GVHD) is currently one of the
serious complications after allogeneic hematopoietic stem cell transplantation
(AHSCT). Many studies have shown the potential role of non-human leukocyte antigen
(non-HLA) gene polymorphism with the incidence of GVHD. In this study, we
analyzed the impact of single nucleotide polymorphism (SNP) within the promoter
region of the interleukin-10 (IL-10) at positions (-1082, -819, and -592), and IL-6 (-
174) on the development of GVHD and overall survival (OS) following pediatric
AHSCT. Method: In this retrospective analysis, we included 94 patients with different
hematological malignancies who received BM (n=80) and PB (n=14) grafts from their
HLA-matched related donors. Most patients (n=85) were treated with myeloablative
conditioning (MAC), whereas the remaining patients (n=9) received reduced-intensity
conditioning (RIC). We used polymerase chain reaction sequence-specific primer
(PCR-SSP) to analyze the SNPs of IL-10 and IL-6 in 94 patients and 92 donors.
Results: In univariate analysis, we did not find a statistically significant association
between the IL-10 genotypes with the incidence of acute or chronic GVHD. Donor IL-6
GG homozygous genotype was found to be significantly associated with the
development of cGVHD (p=0.028). The result of donor IL-6 GG together with other
factors known to affect the risk of aGVHD and cGVHD were subjected to multivariate
logistic-cox regression analysis. This analysis confirmed the association of donor IL-6
GG homozygous genotype to the risk cGVHD (p=0.049). No significant association
was found between IL-10 and IL-6 gene polymorphisms and overall survival (OS).
Conclusion: Our work identified the Donor IL6-174 GG genotype of pediatric AHSCT
as a risk factor for the development of cGVHD.