الفهرس 
Auricular cartilage regeneration using different types of mesenchymal stem cells in rabbits
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Abstract
This study aimed to compare the potential of different types of laser activated
MSCs to promote auricular cartilage regeneration. Twelve adult rabbit
allocated equally in four groups, all animals received a surgical mid auricular
cartilage defect in both ears; group I (Positive control) injected subperichondrially with phosphate-buffered saline (PBS), group II injected
adipose-derived MSCs (ADMSCs), group III received bone marrow-derived
MSCs (BMMSCs), and group IV received ear MSCs (EMSCs). The auricular
defect was analyzed morphologically, histopathologically,
immunohistochemically, and gene expression of the collagen type II (Col II)
and aggrecan after 4 weeks. The auricles of all treatments appeared completely
healed with smooth surfaces and similar tissue color. Histopathologically;
defective areas of control, ADMSCs and EMSCs treated groups experienced
a small area of immature cartilage, while BMMSCs treated group exhibited
typical features of new cartilage formation with mature chondrocytes inside
their lacunae and dense extracellular matrix (ECM). Immunohistochemically;
there was an intense positive S100 expression in BMMSCs, a moderate
reaction in EMSCs, and a mild reaction in the control group and ADMSCs.
The expression of relative col II and aggrecan was substantially highest in
BMMSCs. BMMSCs showed the highest chondrogenic potential compared to
ADMSCs and EMSCs and should be considered the first choice in treatment
of cartilaginous degenerative disorders.