الفهرس 
Pathological Studies on chronic Toxicity of Acesulfame Potassium in Rats
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Abstract
Acesulfame potassium (Ace-K) is one of the commonly used artificial sweeteners, keeping its
sweetening property after heating or freezing encouraging its use in various products especially that
consumed by children, and encounters a potential hazard for cumulative toxicity. Therefore safety
evaluation for its long-term exposure is necessary. A total of 90 mature and immature male Sprague
Dawley rats were divided into two main groups, 45 animals each .Immature group subdivided into 3
groups (G1 control untreated, G3&G5 rats treated with Ace-k at a dose of 15 & 90 mg/kg. b.w) and
the mature group sub divided also into 3 groups (G2 control group, G4&G6 rats treated with Ace-k at
a dose of 15 & 90 mg/kg. b.w), 15 rats per group. All treated rats received Ace-K via gastric
intubation daily for 12 weeks. At the end of the experimental period blood samples were collected for
the determination of serum amylase, lipase, and glycated hemoglobin and also for testing of
lymphocyte proliferation rate, macrophage activity concerning nitric oxide (NO) and the comet assay
for isolated lymphocytes. In addition, pancreas and liver were dissected for histopathological
evaluation. Liver tissues were collected also for the determination of P53 gene expression. Results
revealed that the chronic treatment with Ace-k induced a significant increase in weight gain in
younger age-treated groups compared with older ones. Pancreatic enzymes showed non-significant
differences between control and treated groups at different ages while a significant reduction in
glycated hemoglobin was detected in older age receiving higher doses compared with other treated
groups. Ace-k induced modulation in lymphocyte proliferation rate and affected the production of NO
by macrophage while increases in the tail moment were detected in the isolated lymphocytes in a
dose-dependent manner among different treated groups