الفهرس 
Isolation and biochemical characterization of some siderophores produced by Aspergillus fumigatus
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Abstract
Background: Hydroxamate siderophores are low molecular weight, high
affinity ferric iron- chelating compounds which are excreted by Aspergillus
fumigatus for iron transport extracellularly or iron storage intracellularly.
Methods: Aspergillus fumigatus was isolated from different samples , then
siderophores were extracted from A. fumigatus by phenol, diethyl ether and
purified by amberlite XAD-16 resin. Chemical structure of siderophores was
described by H¹NMR , ESI-MS/MS and LC-MS/MS analysis. Also, the
antimicrobial activity was observed.
Results: from the elution profile of siderophores, the major peak was at
fraction 28 (absorbance 0.98). As a result of H¹NMR analysis, the singlets
which assigned to the -C=O-CH=C(CH3)C-substructure were detected .The
siderophore TafC had antibacterial activity against both G +ve and G -ve
bacteria.
Conclusion: By using different concentrations of iron, it can be deduce that
mycelial fresh weight positively affected by the concentration 0.03 mmol of
iron at 20 h incubation period ,subsequently consequently production of
siderophores increased. from chemical analysis, it was found that the
chemical formula C39H58FeN6O15 was identical to the siderophore N,N’,N”-
triacetylfusarinine C (TafC) which is known.