الفهرس 
Effect of electroporation on liposome characteristics
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Abstract
In the present study, the electroporation technique was used to make
reversible pores through liposomes membrane and subsequently effective
loading of the drug to liposomes. The effect of electroporation on the physical
characteristics of diclofenac sodium (DS), ascorbic acid, and rhodamine B
(Rh-B). loaded 1,2-dipalmitoyl-Sn-glycero-3-phosphocholine (DPPC)
liposome was studied. Ascorbic acid and DS were used as examples of loaded
drugs, and Rh-B fluorescence dye as labeling to visualize the entrapping
liposomes. Samples of DS, ascorbic acid, and Rh-B were divided into three
groups. group one represents liposome vesicles incubated with DS, group two
was liposomes with ascorbic acid and, group three was liposomes with Rh-B.
Liposomes were prepared using the thin-film hydration method. The three
groups were subjected to electroporation protocol. Samples in electroporation
cuvette, were exposed to different field strengths (200, 300, 400, 500, 600, 700,
800, 900, 1000, 2000 and, 3000 V/ 0.2 cm) at pulses 30 pulses, 60 pulses and,
90 pulses and pulse duration 4 milliseconds. After application of the
electroporation protocols, liposomes were separated from unentrapped DS,
ascorbic acid, and dye respectively by centrifugation. Physical characteristics
of liposomes were examined using Transmission Electron Microscopy (TEM),Confocal Laser Scanning Microscopy (CLSM), and Particle size analysis.
Liposomes entrapping ascorbic acid with high efficiency at the values of 900
V/ 0.2 cm with 60 pulses, 800 and 1000 V/ 0.2 cm at 90 pulses. While DS was
entrapped at 800 V/ 0.2 cm and 90 pulses. Rh-B was entrapped at 500 and 1000
V/ 0.2 cm at 30 pulses. Based on the present study, entrapping drugs into
liposomes can be controlled by electroporation technique. Optimum
entrapping occurred at 800 V/0.2 cm and 90 pulses for ascorbic acid and DS
and at 900 V/0.2 cm and 30 pulses for Rh-B.