الفهرس | Only 14 pages are availabe for public view |
Abstract In the present study, a total of 120 Egyptian sole (Solea aegyptiaca) with average body weights (50-70 g) and average lengths (13-19 cm) were collected randomly and seasonally from the Mediterranean-sea in Port Said Governorate, Egypt from September-2019 to August-2020. Fish were found to be infected with two different types of Gram-negative bacteria (Vibrio alginolyticus and Pseudomonas stutzeri) with a total prevalence of 50%. The highest prevalence of infection recorded was due to V. alginolyticus (29.16%) followed by Ps. stutzeri (20.84%). The highest total prevalence of bacterial infection among the naturally infected fish was recorded in summer season (33.34%), followed by spring (26.76%), then winter (25%), while the minimal prevalence of infection was recorded in autumn (15%). Molecular investigation of pure isolates using specific primers were positive to 16S rRNA genes of Vibrio and 16S rDNA genes of Pseudomonas spp. and gave expected amplicons size of 663 bp and 618 bp length, respectively. The histopathology of naturally infected Solea aegyptiaca exhibited severe inflammatory reactions and mononuclear infiltration with activation of melanomacrophage centers as well as tissue necrosis. |