الفهرس 
Cancer
Therapeutic actionOnly 14 pages are availabe for public view
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Abstract
Bee pollen extract (BPE) based polymer nanoparticles (BPENP)
were created in this study by using bovine serum albumin (BSA) and
being targeted with folic acid. Spectroscopic and microscopic methods
were used to characterize the resultant nanoparticles. High flavonoid and
polyphenolic contents were detected by HPLC. At 336 nm, it was found
that BPENP provided the highest absorption by UV. The average particle
size ranged between 25 and 40 nm. The inhibitory effects and IC50
values of the acquired BPE, BPENP, Avastin, or their combination at 24
and 48 hr were detected against A549 lung cancer cells and MCF-7 breast
cancer. The combination therapy assay of BPENP and Avastin revealed a
very significant synergism between the two drugs with a low combination
index (CI). Mice groups are: group 1 received saline, whereas groups 2,
3, 4, 5, and 6 received a single dose of urethane, followed by weekly
injections of butylated hydroxy-toluene (BHT). After the BHT injection,
the mice in groups 3, 4, 5, and 6 received BPE, Avastin, BPENP, and
BNENP + Avastin, respectively. The number and size of tumors
decreased in group 6 compared to those in the other groups. The ratios of
early and late apoptotic cells in Groups 3, 4, 5, and 6 (42.8%, 41.4%,
26.2%, and 45.4%, respectively) were lower than that in the untreated
group by flow cytometry analysis of Annexin v. At the S phase of the cell
cycle, the treatment groups (3, 4, 5, & 6) were significantly lower than
group 2, whereas the combination group 6 were significantly higher than
groups (2& 4). Treatment groups (3, 4, 5, & 6) levels in the mitotic phase
were significantly lower than group 2 levels, whereas combination group
6 levels were significantly higher than individual groups (4& 5).