الفهرس 
Title : Tissue Resident Memory T Cells in Early and Late Cases of Generalized Non-Segmental Vitiligo
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Abstract
Background: Tissue Resident Memory (TRM) T cells have been shown
to play a pivotal role in maintenance of vitiligo. Analysis of the TRM cell
populations, along the course of vitiligo, helps a better understanding of
immunological profile of different stages of vitiligo.
Objective: To detect the presence & diversity of TRM cells in perilesional
and non-lesional skin in early and late generalized Non-
Segmental Vitiligo by immunofluorescent staining of CD 8 and CD 69,
compared to control skin.
Methods: Fifteen patients with short duration vitiligo (duration = or < 6
months) and 14 patients with long duration vitiligo (duration > 6 months)
and 18 healthy controls were included in this study. Clinical evaluation
was done for the patients using VASI and VIDA. Punch biopsies were
obtained from peri-lesional and non-lesional skin of vitiligo patients and
band from skin of normal controls. Immunofluorescent staining was done
using primary antibodies rabbit anti-human CD8 and mouse anti-human
CD69.
Results: CD8 + and CD69+ expression was significantly higher in perilesional
and non-lesional sections compared to controls. On comparing
the short duration and long duration cases, short duration cases
demonstrated a significantly higher VIDA score compared to long
duration cases. CD8+ and CD69+ expression was significantly higher in
the short duration vitiligo patients compared to the long duration patients.
Conclusion: short duration cases of generalized NSV associated with
activity show significantly higher expression of CD8+ and CD69+T cells
which may constitute a TRM cell population capable of inducing
autoimmune memory which might contribute to lesion recurrence after
therapy.