الفهرس 
Title : Molecular and Confocal Laser Scanning Microscopic Evaluation of Mucosal Cells Exposed to Gold Nanoparticles
AbstractOnly 14 pages are availabe for public view
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Abstract
Gold nanoparticles (GNPs) have several biomedical applications such as
photothermal therapy, drug delivery, targeting, and diagnosis of cancers. GNPs
have great compatibility with antibodies, antigens, and other biomolecules.
Therefore, surface functionalization of GNPs can be used to accelerate the
antibody-antigen reaction. In this study, anti-EGFR (Anti-epidermal growth factor
receptor) was conjugated to GNPs then labeled with FITC (Fluorescein
Isothiocyanate). The Anti-EGFR-GNPs-FITC was characterized by UV-VIS, Zeta
potential, and TEM. The Anti-EGFR-GNPs-FITC was applied for targeting tumorinduced
in hamster Mucosal Cells using Confocal Laser Scanning Microscope
(CLSM). CLSM study reveals the successful distribution and GNPs bioconjugation
and accumulation in tumor cells. In addition, a real-time PCR study showed an
increase in mean values of P53 (tumor suppressor gene) expression in both normal
and tumor-induced cells. The up-regulation in gene expression of P53 means using
Anti-EGFR-GNPs-FITC will help in suppressing the tumor cells growth. This
study reveals that Anti-EGFR-GNPs-FITC was successful in molecular and
confocal laser scanning microscopic evaluation of mucosal cells.