الفهرس 
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Abstract
There is limited information regarding acute toxicity and lethal concentration of the antitumor metabolic extract produced by Micromonospora citrea SP1 EMCC 1923. The objective of this study was to evaluate the beneficial effect of the metabolic extract as antioxidant for induced GIT toxicity. Its cytotoxicity on erythrocytes was determined (lethal average concentration, LC 50). The result indicated that, the percentage of haemolysis increased in a concentration-dependent manner of Micromonospora metabolic extract used. The LC 50 value obtained was 69.13 mg / ml.
Results outcome from this experiment suggest that Micromonospora metabolic extract has very low toxicity proving to be safe for treatment. The crude metabolic extract from Micromonospora citrea was also investigated for in vivo cytotoxicity on experimental rats; injection up to 800 mg / kg didn’t cause lethal effects. A systematic investigation of the protective influences of Micromonospora metabolic extract against Diethylnitrosamine / acetylaminoflurene (DEN / AAF) induced gastrointestinal tract toxicity was performed. The
Summary
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rat groups consisted of 8 animals, each. Rats of group 1 (control) received intraperitoneal (i.p.) injection of normal saline (2.5 ml / kg) 3 times per week. group 2 (Micromonospora metabolic extract supplemented) were given the metabolite (5 mg / kg), 3 times per week (i.p.), for 8 weeks. group 3 (chemically induced GIT group) were injected with the same doses of normal saline (3 times per week) for 2 weeks prior to, and 6 weeks after chemical induction of GIT toxicity.