الفهرس 
Title ; Parasitological and Molecular Studies on Cryptosporidium Infecting Claries Gariepinues Fish in Egypt
AbstractOnly 14 pages are availabe for public view
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Abstract
Little known is about the epidemiology of Cryptosporidium in fish. This study scopes
out the prevalence and molecular detection of Cryptosporidium spp. in cat-fish (Clarias
gariepinus). A total of 300 Carias gariepinus fish were collected from two fresh water
sources; River Nile (180) and drainage canals (120). Stomach and intestine epithelium of each
individual fish samples were screened with staining technique of modified Ziehl-Neelsen
(mZN) for recognition of Cryptosporidium oocysts followed by Enzyme Linked
Immunosorbent Assay (ELISA) for detection of Cryptosporidium antibodies and molecular
characterization using complemented DNA polymerase chain reaction (cPCR). ELISA
showed higher prevalence of 69.3% than that prevalence obtained by mZN, 64% for the total
examined Clarias gariepinus fish.