الفهرس يوجد فقط 14 صفحة متاحة للعرض العام
 |  | from | 174 |  |  |
| | | from | 174 | | |
المستخلص
B. cepacia (B. cepacia) has recently received a considerable attention as one of the major risks in susceptible pharmaceutical products. This microorganism can easily propagate and cause vast and severe contamination especially to the water supplies for pharmaceutical companies. Moreover, it proliferates within the products and can cause severe infections for humans. Therefore, fast and sensitive detection of these bacteria is of a great demand. The present study introduces improved application of PCR assay with relatively high sensitivity and specificity for the direct detection of B. cepacia from the aqueous pharmaceutical products. A semi-nested PCR (SN - PCR) approach using the primer set BCR1 / BCR2 followed by BCR1 / Mr yielding a 465 - bp fragment of the recA gene was applied and tested using both crude lysate from isolated colonies and DNA directly extracted from artificially prepared and spiked syrup. The PCR assay showed no interference with other bacterial reference and environmental strains tested including: Staphylococcus aureus ATCC® 6538, pseudomonas aeruginosa ATCC® 9027, Escherichia coli ATCC® 8739, salmonella abony NCTC® 6017 and Bacillus subtilis ATCC® 6633, Micrococcus luteus, staphylococcus warneri, pseudomonas fluorescens, pseudomonas putida and Ralstonia pickettii