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Abstract
Cardiac diseases are among the main causes of morbidity and mortality. Despite significant advances in medical and interventional therapy, cell replacement is considered as a promising therapeutic modality for cardiac diseases. hiPSCs offer the potential to generate large numbers of functional cardiomyocytes from patient-specific cell sources bypassing ethicalissues and the immunological rejection ofother stem cells sources. The most successful differentiation approaches are those recapitulatingthe regulatory pathways that control the establishment of the corresponding lineage in the early embryo.The aim of the study was to differentiate NP0040 hiPSC line into cardiomyocytes andto show evidence of cardiac differentiation. Cardiac differentiation was done by temporally modulating the regulatory elements of the signaling pathways: wnt, BMP-4, FGF&ascorbic acid in a monolayer-based culture system under serum-free, feeder-free conditions with subsequent purification by 2Lactate method3. characterization of the generated cardiomyocytes was done by flow cytometry, immunofluorescence,reverse-transcriptase PCR and electrophysiological studies.Here we showed thattemporal modulation of wnt signaling is essential for mesoderm induction and cardiac specification (protocol (A)