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Abstract
One hundred and fifty positive serum samples for mycoplasma using serum plate agglutination test (SPA) were collected .The included samples were one day old chicken (36 samples ), broilers (40 samples),layers (38 samples), balady chicken (14 samples) and turkeys (22 samples).The samples were collected from different farms in El- Kaliobia, Eldakahlia, El behera and Beni-seuif governorates and examined To study the incidence of MG in the respiratory tract. Samples from nasal cavity, ifra-orbital sinus, trachea, air-sacs and lung were collected from the examined birds. were cultivated in mycoplasma media containing sterile filtrated swine serum and using also horse serum to detect Mycoplasma gallisepticum. All suspected colonies were identified by conventional method, growth inhibition test and PCR.