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Abstract
Listeria monocytogenes is considered one of the most important food-borne pathogens that is transmitted to human mainly via contaminated food. In an attempt to throw more light on zoonotic importance of L. monocytogenes as food-borne pathogen, a total of 340 samples were used for this study; these samples included 250 food samples (50 beef burger, 50 sausages, 25 Luncheon, 25 minced meat, 50 raw milk and 50 kariesh cheese) were randomly collected from retail markets, groceries and restaurants in different localities in El Giza Governorate. In addition swabs were taken from 40 food refrigerators and stool samples from 50 diarrheic children. All samples were cultured for the isolation of L. monocytogenes using the conventional International Organization for standardization (ISO, 11290-1) method. The isolates were tested biochemically using Listeria Microbact 12L and confirmed by PCR. The results revealed that the isolation rates of L. monocytogenes were 8 %, 4%, 4% for beef burger, minced meat and Luncheon respectively, while sausage samples were totally negative