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العنوان
In vitro comparative study of the antibacterial effect of lactobacillus acidophilus and antimicrobial agents on different isolates in burns /
الناشر
Marian Lotfy Aziz Ayad ,
المؤلف
Marian Lotfy Aziz Ayad
هيئة الاعداد
باحث / Marian Lotfy Aziz Ayad
مشرف / Mona Abdelwahab Abdelmessih
مشرف / Amal Ali Ali Elshimy
مشرف / Hamed Mohamed Kadry
تاريخ النشر
2015
عدد الصفحات
125 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
علم المناعة والحساسية
تاريخ الإجازة
11/5/2015
مكان الإجازة
جامعة القاهرة - كلية الطب - Microbiology and Immunology
الفهرس
Only 14 pages are availabe for public view

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from 135

Abstract

Material and Methods: Burn wound swabs were obtained from 50 patients who had been admitted to Burns Unit in Kasr El-Einy Teaching Hospital. These swabs were inoculated onto enriched and differential culture media. Subcultures were performed on selective media. The necessary biochemical tests were conducted and the organisms identified using standard procedures. Susceptibility of isolated pathogens to Lactobacillus acidophilus supernatant and the commonly used burn wounds antibiotics was examined using standard susceptibility testing. Results: Ninety five different organisms were isolated. Klebsiella spp.38 (40%) were the most commonly isolated organisms, followed by Staphylococcus aureus (27.4%), Pseudomonas spp., (14.7%)Proteus spp.(8.4%), E.coli (6.3%), MRSA (2.1%), Acinetobacter spp.(1.1%) respectively. In susceptibility testing, LAB supernatanthad an inhibitory effect against 57 isolates (60%). The inhibitory effect of the remaining antibacterial agents used was different in their activity according to the nature of the isolate (resistance or sensitivity). Conclusion: The results of the study concluded that Lactobacillus acidophilus supernatant in undiluted formhad a high activity to inhibit the growth in-vitro of 60% of the pathogenic gram-positive and gram-negative bacteria, which cause burn wound infections. This indicated the therapeutic efficacy of Lactobacillus acidophilus bacteria