الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 151 |  |  |
| | | from | 151 | | |
Abstract
Background: The resistance of E. coli isolates to
fluoroquinolone is a significant challenge to antibiotic treatment and
infection control policies. A significant increase of ciprofloxacinresistant
E. coli has occurred during last years, indicating the need for
further analysis.
Objective: The current study was aimed to detect quinoloneresistance
in multi-drug resistant E. coli isolates that were recovered
from patients with urinary tract infection in the Urology and
Nephrology center of El-Mansoura University, Egypt.
Methods: The clinical uropathogenic specimens collected were
studied. Identification and antimicrobial susceptibility testing were
done using VITEK 2. Minimum inhibitory concentrations (MICs) of
different quinolones were determined for fluroquinolone resistance
(FQ-resistant) isolates. Also, they were screened for the presence of the
plasmid mediated quinolone resistance (PMQR) qnrA, qnrB and qnrS
genes by polymerase chain reaction (PCR) techniques.
Results: In the present study, 100 (32.7%) E. coli of 305
uropathogenic specimens were tested. The patients involved in this
study included both genders, females (76%) were the most affected
group of patients as compared to males (24%). The quinolones
resistance rate was 30% of the isolated E. coli samples. The detected
genes for the quinolone’s resistance samples were: 14 (46.6%) qnrB,
13 (43.3%) qnrS and 2 (6.6%) qnrA.
Conclusion: This study identified quinolone resistance (qnr) gene in
uropathogenic E. coli in EGYPT. These finding which suggest a
possible resistance to quinolone are of high interest for better
management of patients and control of antimicrobial resistance in
Egypt.