الفهرس 
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Abstract
Water pollution is such a serious environmental concern these days that it requires serious attention. The water is polluted due to heavy discharges of industrial effluents that contain organic waste. Various techniques, including physical, chemical and biological, have been used to treat wastewater, and these methods have various disadvantages that produce a large amount of sludge. nanotechnology has been widely applied in all branches of science and technology, thus various materials have been prepared and used to remove water pollutants and more attempts have been made to discuss various aspects of water treatment by adsorption using NPs. In this work six types of mono and bimetallic NPs of iron, copper and zinc metals were biologically synthesized by using F. oxysporum. The produced mono and bimetallic NPs were charachterized to detect their characteristic absorption peak, size, shape and crystal structure of NPs. The optimal condition for the biosynthesis of mono and bimetallic NPs were investigated to reach the maximum production of NPs. Antimicrobial activity of biosynthesized mono and bimetallic NPs were investigated against 5 types Gram +ve, 4 types Gram –ve bacteria and one fungus Candida sp. Fe2O3 NPs did not have any inhibition activity aganist all tested strains while CuO NPs has inhibited the growth of all tested strains and also ZnO NPs showed inhibition activity aganist the tested pathogens except Klebsilla sp and Bacillus cereus. The produced bimetallic NPs showed full inhubition for all the tested Gram +ve, Gram –ve and Candida sp. The MIC of mono and bimetallic NPs were were variable with the tested pathogenic strains. The ability of mono and bimetallic NPs to adsorb CR and MB dyes molecules has been investigated.The kinetic and equilebrium isotherm have been studied for dye removal by NPs, where the kinetic studies for the removal of CR and MB fitted to pseudo-second-order and equilibrium studies fitted to Langmuir isotherm model. The optimal conditions for MB and CR removal by mono and bimetallic NPs have been investigated using CCD. The maximum removal of MB by Fe2O3, CuO, ZnO, Fe2O3\CuO, Fe2O3\ZnO and CuO\ZnO NPs were found to be 91%, 75%, 96%, 85%, 92% and 96% respectively, while the maximum removal of CR by Fe2O3, CuO and Fe2O3\CuO were found to be 98%, 94% and 99% respectively. The toxicity of NPs was investigated by studying the effect of NPs suspension on seed germination of Vicia faba. The results indicated that the NPs do not cause any additional stress to the plant system.In conclusion, F. oxysporum is considered as a good bio-factory synthesizing the bimetallic NPs that have better activity than monometallic NPs against pathogenic bacteria and fungi as well as being effective in the removal of Methyl Blue and Congo Red from polluted water. So, bimetallic NPs could be considered as good nanomaterials for controlling disease and wastewater treatment.