الفهرس 
Comparison Between Er, Cr: YSSG Laser and Conventional etching Methods as Surface Treatment For Pits Fissure Sealants Regarding Microleakage and Adaptation: An In Vitro Study
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Abstract
The current study aimed to compare ER, CR: YSSG laser and conventional acid etching when used
as surface treatment of pits and fissures on occlusal surface of premolar regarding microleakage,
marginal adaptation, penetration, and retention of pits and fissure sealants.
Twenty-six sound premolars with deep pits and fissures were extracted for orthodontic treatment were
included in the study. Teeth were cleaned from any gross debris and were disinfected using sodium
hypochlorite. Teeth were stored in distilled water in a sealed sterile glass container and were divided
randomly into two equal groups (n=13), group I was pretreated with ER, CR: YSSG laser, and Group
II was pretreated with conventional acid etch before the application of fluoride-releasing resin-based
pits and fissure sealant.
All samples were subjected to thermal cycles in water baths at a temperature range between 5°C and
55°C. The duration of each bath was 15 s with a 10 s transfer time. The thermal cycles were repeated
1000 times and a dwell time of 5s then sectioning in a buccolingual direction into two halves in a
vertical plane parallel to the long axis of the tooth. Sealant retention, penetration, marginal adaptation,
and microleakage were evaluated for both groups
Numerical data were summarized using mean, standard deviations and were analyzed using the
student t-test with the value of P < 0.05 was considered statistically significant. Categorical data were
expressed as numbers and percentages and were analyzed using the chi-square test.
Both groups showed a similar result regarding sealant retention and penetration percentage with no
statistically significant difference. ER, CR: YSSG laser surface treatment showed higher marginal
adaptability scores and lower microleakage scores compared to conventional acid etching with a
statistically significant difference between both groups.
Both ER, CR: YSSG laser surface treatment, and conventional acid etching are promising techniques
for surface treatment before the application of pits and fissures sealants providing a 100% complete
retention of the sealants. Both conventional acid etching and ER, CR: YSSG laser surface treatment
allowed penetration of sealants nearly to the full depth of the fissures. ER, CR: YSSG laser surface
treatment showed higher adaptation of the pits and fissure sealant compared to conventional acid
etching. However, both groups showed a similar percentage of the dye penetration, ER, CR: YSSG
laser surface treatment showed significantly lower microleakage.