الفهرس 
Interplay between hepatoma cells and fibroblasts : A potential role of Syndecan-1
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Abstract
The expression pattern of syndecan-1(SDC-1) in Hepatocellular carcinoma
(HCC) has been changed from normal sinusoidal to constant honeycomb-like
membrane distribution. Angiotensin II (Ang II) was found to relate to liver fibrosis
development. TGF-β1 is mainly derived from infiltrating monocytes and induces the
progression of liver fibrosis. The present study aimed to investigate the interaction
between hepatic cancer cells, fibroblasts, and monocytes in the presence of
overexpressed SDC-1. The human hepatocellular carcinoma (Hep3B), Syndecan-1
transfected Hep3B (Hep3B-SDC-1), monocyte cell lines (U937 and Mono-Mac-6), and
human hepatic stellate (LX2) cell lines were cultured in vitro. Secretomes were detected
using the dot blot technique and cytokine array. The results showed alteration in
secreted proteins, cytokines, chemokines, basal expression levels, and activation status
of multiple signaling pathways of Hep3B-SDC-1 compared to vector cells. In addition
to the alterations detected in various signaling pathways in monocytes cell lines upon
stimulation with Secretome of Hep3B, Hep3B-SDC-1, and Lx2 cells. Ang II and TGFβ1 influence Lx2 cells. Significant decrease in AT-1 expression in cirrhotic liver tissue
due to HCV was observed with significant elevation in SDC-1 in liver tissues with
cirrhosis and HCC. In conclusion, the presented results suggest that SDC-1
overexpression induces specific alterations in tumor-microenvironment associated cells
such as (fibroblasts and monocytes). Furthermore, the interaction of SDC-1 with Ang II
and TGF-β has been revealed to induce a critical modulation in hepatic cancer cells and
fibroblasts.