الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 147 |  |  |
| | | from | 147 | | |
Abstract
ABSTRACT
This study was designed to investigate the efficacy of MSC-MVs, A-PRF, and I-PRF to enhance healing and regeneration of experimentally induced full thickness skin defect in equine model. Sixteen adult healthy donkeys of native breed of both sexes (weighing 150-250) were included in the study. Two full thickness skin wounds (3 x 3 cm) were created on each side of the back region. In all animals, the cranial wounds at the left side were daily irrigated using 2.5% Povidone-iodine, while the caudal ones were irrigated using normal saline. According to the used treatment for right side wounds, these donkeys were randomly allocated into four equal groups. In first group, the wounds were treated via subcutaneous and intradermal injection of 4ml MSC-MVs solution at day zero and repeated at 14th day PW. In second and third groups the wounds were treated via topical application of MSC-MVs gel and A-PRF membrane respectively at day zero and repeated at 2nd, 4th, and 7th day PW. In fourth group, the wounds were treated via subcutaneous and intradermal injection of 4 ml of I-PRF solution at day zero and repeated at 14th day PW. Clinical evaluation of the treated wounds was performed by digital photographs accompanied by estimation of the rate of wound contraction at day 0, 3, 7, 14, 21, 28, 35, and 42 PW. Histopathological and immunohistochemical evaluation were performed at 2nd, 4th, and 6th weeks PW. A scoring system was used for histological assessment of wound healing. Immunohistochemical evaluation of CD31 and alpha smooth muscle actin (α-SMA) markers for angiogenesis were detected by immunoperoxidase technique. Clinical evaluation revealed that wound contraction started at the 14th day PW and proceeded rapidly till the end of the study (42nd day PW). I-MVs showed the highest rate of contraction followed by I-PRF, T-MVs, and finally A-PRF group when compared to control wounds. Histopathological evaluation showed the superiority of I-MVs, T-MVS, and I-PRF compared to and A-PRF group and control wounds. Immunohistochemical evaluation revealed that I-MVs, T-MVs, and I-PRF treated wounds showed higher immunoreactivity against CD31 and α-SMA antigens throughout the study period compared to A-PRF and control wounds. In conclusion, the present study is considered as a prelude for future use of MSC-MVs and I-PRF biomaterials for management of equine wounds.
Keywords: A-PRF, Equine, I-PRF, Mesenchymal Stem Cells, Microvesicles, skin wounds.