الفهرس 
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Abstract
A total of 500 samples of various origins; 400 fishes Tilapia niloticus, Mugil cephalus, catfish & carp fishes),
50 pond water & 50 human (skin swab fisher men) samples were collected from (private and governmental
farms) from different localities within Suez Canal area for phenotypic and genotypic identification of some
potential toxigenic fungi, as well as molecular characterization of aflatoxigenic fungi in addition to aflatoxin
analysis using ELISA technique. The findings revealed that the frequency distribution of total fungal isolates
were 83 % from fishes, 13 % from pond water and 4 % from human. Surface samples showed the highest
percentage of isolates followed by gills and internal organs., four fungal genera were recovered. Aspergillus
spp. (71%) followed by Penicillium spp. (18%) with low occurance of Mucor spp. (7%) and Alternaria spp.
(4%). all A.flavus tested isolates were positive by 100% to omt-1 & ver-1 genes, while A.parasiticus isolates
was positive by 84% both aflatoxigenic genes (omt-1 & ver-1).most of samples contain aflatoxin with variable
concentrations below the permissible limits.