الفهرس 
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Abstract
This study was conducted in the Medical Biochemistry department in collaboration with the Psoriasis Outpatient Clinic of the Department of Dermatology, and Venerology, Faculty of Medicine at AUH in the period between July 2021 and March 2022. It was conducted to identify the role of GLUT 1 genes expression in the pathogenesis and severity of psoriasis disease. This study also aimed to: Compare GLUT 1 relative expression among psoriatic patients, psoriatic with T2DM patients, and healthy individuals. Correlate the GLUT 1 relative expression with the severity of psoriasis disease using psoriasis area and severity index (PASI) score Measure and compare serum PEDF levels in psoriatic patients, psoriatic with T2DM patients, and healthy individuals Test the effect of in vitro treatment of human skin fibroblast (HSF) and Hep G2 with PEDF on GLUT 1 expression. Our studied participants are divided into three groups: healthy control group, psoriatic group, and psoriatic with T2DM group. Psoriatic patients with T2DM was the oldest group. Males formed the majority of the three studied groups. The BMI was significantly higher in psoriatic with T2DM than other two studied groups. There was a non- statistically significant difference between psoriatic and psoriatic with T2DM regarding PASI score. Liver function tests, kidney function tests, and lipid profile were within the normal ranges in the three studied groups, while the mean random blood glucose, and glycated hemoglobin percentage were significantly higher in psoriatic with T2DM group. ALT, serum LDL-c, and serum total cholesterol were significantly higher in psoriatic group, while AST, and serum triacylglycerol were significantly higher in psoriatic with T2DM group. The whole blood GLUT 1 mRNA relative expression was significantly the highest in psoriatic group, followed by psoriatic with T2DM group, then healthy control. The serum PEDF level was significantly the highest in control group, followed by psoriatic with T2DM group, then psoriatic group. There is statistically significant difference between different PASI score categories regarding whole blood GLUT 1 mRNA relative expression. The cut-off point of whole blood GLUT 1 relative expression was 4.7 to differentiate psoriatic from normal subjects. While the cut-off point of serum PEDF level was 26.6 ng/ml to differentiate normal subjects from psoriatic patients. There is a significant positive correlation between whole blood GLUT 1 mRNA relative expression and each of duration of psoriasis and its severity (PASI score). There is a significant negative correlation between serum PEDF and each of duration of psoriasis, duration of T2DM, and whole blood GLUT 1 mRNA relative expression.GLUT 1 was not expressed in human skin fibroblast. In vitro treatment of Hep G2 cell line with PEDF decreased GLUT 1 mRNA relative expression compared to untreated one. We have shown for the first time a statistically significant association between the base line mRNA relative gene expression and the severity of psoriasis reactions . Our finding suggests that GLUT 1 mRNA relative gene expression levels higher than cutoff values devised may predict moderate to severe allergic reactions in patients with psoriasis. There is statistically significant difference between different PASI score categories regarding whole blood GLUT 1 mRNA relative expression. The cut-off point of whole blood GLUT 1 relative expression is 4.7 to differentiate psoriatic from normal subjects. While the cut-off point of serum PEDF level is 26.6 ng/ml to differentiate normal subjects from psoriatic patients. There is a significant positive correlation between whole blood GLUT 1 mRNA relative expression and each of duration of psoriasis and its severity (PASI score). There is a significant negative correlation between serum PEDF and each of duration of psoriasis, duration of T2DM, and whole blood GLUT 1 mRNA relative expression. We also have identified a strong connection between the serum levels of PEDF and psoriatic patients. We have identified a clinicioimmunological effect at cellular levels. As we have shown the effect of PEDF on GLUT 1 relative expression in Hep G2 cell.