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Abstract
An 84 specimen collected from 42 of sound extracted human molar teeth were used in this study. The teeth were sectioned in a bucco-lingual direction into mesial and distal halves using a diamond cutting disc at low speed hand piece under water coolant; then the mineral content of each specimen was measured using Energy Dispersive analysis X-Ray spectroscopy (EDAX) to obtain the baseline measurement. After that the teeth were immersed in a demineralizing solution in a proportion of 2ml solution/mm2 for 72 hours to prompt caries like lesion (white spot lesion), then the mineral content of demineralized specimen were measured again by EDAX to detect the response of enamel surface to acid attack. All Specimens were randomly divided into three main groups according the remineralizing paste used with 28 specimens each, where the first is demineralized enamel treated with fluoride, the second demineralized enamel treated with CPP-ACP with fluoride and the third is demineralized enamel treated with Novamin with fluoride. Each group was divided into two subgroups according to pH cycling application with 14 specimens each, where the first subgroups the specimen subjected to PH cycling and second represent without pH cycling. In order to standardize volume and application time of all tested remineralizing pastes, a 20 units of all tested tooth paste were applied to enamel surface was using insulin syringe then the paste were rubbed for 5 minutes on specimen surface by a micro brush then rinsed off with water and dried with air syringe