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Abstract
The present work aimed to study the effect of inoculation Pimpinella anisum L. seeds with biofertilizer [arbuscular mycorrhizal fungi (my) and/or microbein (mi) ] and/or plants were spray with Thidiazuron (TDZ) combined with chemical fertilizer at half or full dose of NPK on number of spores Am fungi per kg soil, AM fungi colonization , enzymatic activities (dehydrogenase & Nitrogenase), growth parameters , chemical composition and active constituents . Also, some molecular studies (protein electrophoresis) were studied. This work was carried out during two successive seasons 2012-2013 and 2013-2014 at Biotechnology Department, Phytochemistry Department and Farm of Applied Research Center of Medicinal Plants (ARCMP) affiliated to the National Organization for Drug Control And Research (NODCAR), ministry of health, Egypt. The most important results could be summarized as follow: The results in both seasons showed that, the highest values of number of AM fungi spores per kg soil in anise (Pimpinella anisum L.) roots, AM fungi colonization %, enzymatic activities, growth parameters, chemical composition and active constituent obtained at inoculated seeds with mixture of mycorrhizal spores and mycrobein at full dose of NPK. The profile of SDS-PAGE showed appearance and disappearance of some protein bands occurred after chemical , bio fertilizers and foliar sprayed by TDZ of anise plant compared to the control. The highest number of bands (up to 19 bands) were observed with the inoculated anise (Pimpinella anisum L.) seeds with mixture of mycorrhizal and microbein