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العنوان
Some studies on Salmonella infection in sheep and goats /
الناشر
Manar Magdy Farouk Sayed Abdelshafy ,
المؤلف
Manar Magdy Farouk Sayed Abdelshafy
هيئة الاعداد
باحث / Manar Magdy Farouk Sayed Abdelshafy
مشرف / Amal Abd El-Aziz El-Molla
مشرف / Diea G. Abo El Hassan
مشرف / Adel Ibrahim Tanios
تاريخ النشر
2017
عدد الصفحات
113 P. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
تاريخ الإجازة
15/1/2017
مكان الإجازة
جامعة القاهرة - كلية الطب البيطري - Medicine and Infectious Diseases
الفهرس
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Abstract

Diarrhea is a major problem of livestock in Egypt and throughout the world. Salmonella is one of the main etiological agents of infectious diarrhea and represents a zoonotic hazard worldwide. In the current study, a total number of 197 and 149 diarrheic sheep and goats of different age groups, at different seasons were clinically examined and from which rectal swabs were collected. Rectal swabs were bacteriologically examined for isolation of Salmonella. There were 5 Salmonella isolates recovered from diarrheic goats (3.36%) and 3 from diarrheic sheep (1.52%). The highest rate of Salmonella infection among the examined diarrheic sheep and goats was in Spring season and in (1-2.5 years) age group. Serological identification of the recovered Salmonella isolates revealed 6 different serotypes (Salmonella Bonariensis, Salmonella Kottbus, Salmonella Stanleyville, Salmonella Enteritidis, Salmonella Paratyphi A and Salmonella Ferruch) and 2 Untypable isolates. The recovered isolates were examined for their antimicrobial sensitivity and it was found that the highest resistance rates were recorded against ampicillin, amoxicillin, levofloxacin and ciprofloxacin with resistance rates 100%, 62.5%, 62.5% and 50%, respectively. While the highest sensitivity rates were detected for cefotaxime, gentamycin, amikacin, streptomycin, norfloxacin, sulphamethaxozle+trimethoprim, chloramphenicol and enrofloxacin with sensitivity rates 87.5%, 87.5%, 75%, 75%, 62.5%, 62.5% ,62.5% and 50%, respectively. PCR was applied for genotypic characterization of virulence genes (invA, Stn, bcfc and avrA). All isolates were positive for the 4 virulence genes. Also, PCR was used for confirmation of the existed resistance pattern in antibiogram test