الفهرس 
Title : Studies on the effect of gold nanoparticles on diagnostic polymerase chain reaction PCR technique
AbstractOnly 14 pages are availabe for public view
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Abstract
Polymerase chain reaction (PCR) technique is considered to be one of the most highlighted and promising applications in molecular diagnostics. Conventional PCR could results in false negatives due to low nucleic acid copy number in low titer samples which rises the necessity to improve the technique{u2019}s efficiency. In this study, we used equine Herpes Virus-1 (EHV-1) DNA as a template to determine the effect of 15 nm unmodified gold nanoparticles (GNPs) on the key PCR reactants. The overall reaction outcomes has been enhanced after the optimization of the reaction regarding the GNPs, primers and taq polymerase concentrations for more efficient, highly sensitive molecular detection of EHV-1. The optimized GNPs assisted PCR resulted in specific high yield amplification with a detection limit of 100 DNA copies. The sensitivity of the optimized technique was 92.3% in comparison to 68.6% of conventional PCR which permit the EHV-1 detection in ” " ~ " ”11% more samples than conventional PCR