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Abstract
A feeding trial was conducted to evaluate the impact of dietary supplementation of thermo-stable phytase enzyme (quantum blue®) and a new analogue of Vitamin D₃ (AlpHÜD₃®) singly or in combination on growth performance, bone mineralization, carcass traits and blood parameters in broiler chickens. A total of 720- unsexed, 1-d-old ross 308 broilers were allocated randomly into six equal groups, 3 replicates each. The experimental period extended for 33 days. The six dietary groups were: the first group served as positive control and fed diet containing an adequate levels of available phosphorus (av. P) and calcium, while, the second group fed diet containing 30% av. P and calcium requirements (negative control). The third and fourth groups were fed on diet containing the same levels of av. P and calcium as in the negative control one but supplemented with thermo stable-phytase enzyme (quantum blue®) 500 and 1000 FTU/kg diet respectively. The fifth group was fed on diet containing the same levels of av .P and calcium as in the negative control but supplemented with 5 ppb of AlpHÜD₃®, while group six was fed on diet containing the same levels of av. P and calcium as in the negative control but supplemented with 500 FTU/kg phytase in addition to 5 ppb of AlpHÜD₃®. Results of the experiment showed that the dietary supplementation of phytase enzyme or vitamin D₃ significantly improved (P{u2264}0.05) broiler chicken body weight gain, feed conversion ratio, serum Ca and ALP, carcass traits and tibia Ca/p compared with those fed un-supplemented diets. In addition, it can be concluded that, the use of thermo-stable phytase enzyme in broiler diets at 500 FTU/kg of feed along with vitamin D₃ supplementation at 5ppb resulted in better growth performance and carcass traits, improved Ca/P levels in tibia ash and Ca levels in the blood of broiler chicken