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Abstract
Regenerative medicine aims at helping the body to form new functional tissue to replace lost or defective ones. Human body has an endogenous system of regeneration through stem cells, where stem cells are found almost in all type of tissue. Mesenchymal stem cells (MSCs) represent an archetype of multipotent somatic stem cells that hold promise for application in regenerative medicine. The present study aimed to isolate MSCs from adipose tissue and amniotic fluid (hAFSCs) and differentiate into adipocyte cells. Adult human adipose tissue (hATSCs) contains a population of mesenchymal stem cells, which can be harvested readily, safely, and in relative abundance by modern liposuction techniques. MSCs were separated from adipose tissue liposuction and amniotic fluid, cultured in dullbecus modified egal media (DMEM) for two weeks for proliferation of MSCs, which recollected and regrowing in specific media for differentiation of adipocyte cells (ACs). ACs were determined by staining with oil-red O and RT PCR assessments of lipoprotein lipase and adiponectin genes. Our findings revealed that the MSCs derived from amniotic fluid cells showed high capacity of differentiation into adipocytes comparing with that derived from adipose tissue. The ACs derived from hAFSCs were more prominent and characterized by reddish brown-droplets following staining with oil red stain. Both types of adipocyte stem cells derived from either hATSCs or hAFSCs showed similar expression of molecular bands of adponectin and PPAR-Þ gene. The conclusion that adipocytes derived from MSCs of hAFSCs were markedly growing and expanded comparing with that hATSCs for application in regenerative medicine