الفهرس 
The use of adaptive optics to improve image quality of the wide-field transmission microscopy
ContentsOnly 14 pages are availabe for public view
 |  | from | 130 |  |  |
| | | from | 130 | | |
Abstract
Imaging is a very important diagnostic modality in medicine and other applications. Optical imaging is of superior importance in medicine because of its safety and direct production of spatial images without the need to reconstruction techniques. Unfortunately, there are many problems associated with optical imaging such as the problem of aberration. Many trials have been conducted to correct the aberration and improve image quality. Recently, the adaptive optics (AO) has been investigated for this purpose. The aim of this work is to use AO to correct the aberration and hence improve the quality of the images produced by wide-field transmission microscope. We used a thorlab adaptive optice tool kit to build a modified optical path of the wide-field microscope. The wavefront (image) is captured by the shake Hartmann sensor (SHWS) to the computer. An algorithm for measuring the aberrations and calculating the correction factors was applied to this wavefront. The correction factors are then fed to the deformable mirrors (DM) part of the kit to modify its tilting and hence compensate for wavefront deformation. The modified setup was tried with different tissue samples (blood sample, ileum of rabbit and brain of snack). Four different images with nineteen image details are used in this work. The sharpness of the image details and the overall contrast of the images before and after correction are used to measure the improvement. Observation of new image details and spatial shift were also reported as indications of aberration correction. The obtained results revealed an average sharpness of 3.533 before correction and 8.593 after correction. The overall contrast increased from an average of 1.254 to 1.688. Also, new details appeared in some images and nonlinear spatial shift of details were also observed which ensures the effectiveness technique